A method for the simultaneous isolation of factor X and prothrombin from bovine plasma.
نویسندگان
چکیده
1. A method is described for the simultaneous isolation of both Factor X and prothrombin from bovine plasma. The proteins are adsorbed on and eluted from barium sulphate and chromatographed on DEAE-Sephadex and are finally purified by rechromatography on DEAE-Sephadex. 2. The proteins can be purified in 48h from the collection of the blood and the method can be used to process large volumes of plasma. 3. The prothrombin has a molecular weight of 70300; the Factor X, on the other hand, is polydisperse, with most of the protein (86%) having a molecular weight of 56000.
منابع مشابه
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ورودعنوان ژورنال:
- The Biochemical journal
دوره 131 4 شماره
صفحات -
تاریخ انتشار 1973